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Reference sheet · Enzyme Substrates · CPC catalog code CASP-009
Full physicochemical data (sequence, molecular formula, molecular weight, CAS) is documented on the upstream certificate of analysis. Request the current lot COA from the supplier before use — values may vary between manufacturing runs.
Reference standards in the Enzyme Substrates class are typically shipped as lyophilized powder and stored desiccated at −20 °C protected from light. Once reconstituted in bacteriostatic water or the assay-appropriate buffer, aliquot and store at −80 °C to minimize freeze–thaw cycles. Confirm handling requirements against your institutional biosafety and radiation protocols before use.
Ac-Tyr-Val-Ala-Asp-AFC sits within the Enzyme Substrates research class (CPC reference CASP-009). The peptide encodes a recognition sequence flanked by a reporter group (pNA, AMC, MCA, FAM/DABCYL). Enzyme cleavage releases the reporter, producing a quantifiable optical signal proportional to catalytic activity. Investigators typically incorporate Ac-Tyr-Val-Ala-Asp-AFC as a reference standard, tool ligand, or substrate in the assay contexts listed above. Read on kinetic plate readers with the reporter-appropriate filter set (e.g. 405 nm, 380/460 nm).
Ac-Tyr-Val-Ala-Asp-AFC (CPC catalog code CASP-009) is a synthetic enzyme substrate (research reagent) in the enzyme substrates category. It is provided as a lyophilized ~1 mg vial for in-vitro research use only.
Published and internal research programs use Ac-Tyr-Val-Ala-Asp-AFC as a reference standard in enzyme substrates workflows — for example, kinetic (kcat / km) characterization of proteases and hydrolases and high-throughput inhibitor screening. Human clinical evidence is not established.
No. Ac-Tyr-Val-Ala-Asp-AFC is a research-grade reference peptide and is not approved by the FDA or any national regulator for human or veterinary therapeutic use. It is offered exclusively for in-vitro laboratory research and does not ship to the United States for consumer use.
In a enzyme substrates workflow, Ac-Tyr-Val-Ala-Asp-AFC is reconstituted in sterile buffer or DMSO (solubility-dependent) and applied in the researcher's chosen assay format. Read on kinetic plate readers with the reporter-appropriate filter set (e.g. 405 nm, 380/460 nm).
Other reference standards in the same class — commonly cross-referenced during assay design and comparator selection.