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Reference sheet · Protease Substrates · CPC catalog code MMPS-028
Reference standards in the Protease Substrates class are typically shipped as lyophilized powder and stored desiccated at −20 °C protected from light. Once reconstituted in bacteriostatic water or the assay-appropriate buffer, aliquot and store at −80 °C to minimize freeze–thaw cycles. Confirm handling requirements against your institutional biosafety and radiation protocols before use.
Ac-Pro-Leu-Ala-[(S)-2-mercapto-pentanoyl]-Trp-NH2 sits within the Protease Substrates research class (CPC reference MMPS-028). The peptide encodes a specific protease-recognition motif flanked by a reporter (pNA, AMC, MCA / Dnp FRET pair). Enzymatic cleavage releases the reporter, producing signal directly proportional to catalytic activity. Investigators typically incorporate Ac-Pro-Leu-Ala-[(S)-2-mercapto-pentanoyl]-Trp-NH2 as a reference standard, tool ligand, or substrate in the assay contexts listed above. Read on kinetic plate readers at the reporter-appropriate wavelength.
Ac-Pro-Leu-Ala-[(S)-2-mercapto-pentanoyl]-Trp-NH2 (CPC catalog code MMPS-028) is a synthetic protease substrate (research reagent) in the protease substrates category. It is provided as a lyophilized ~1 mg vial for in-vitro research use only.
Published and internal research programs use Ac-Pro-Leu-Ala-[(S)-2-mercapto-pentanoyl]-Trp-NH2 as a reference standard in protease substrates workflows — for example, kinetic (kcat / km) protease characterization and inhibitor screening and ic₅₀ determination. Human clinical evidence is not established.
No. Ac-Pro-Leu-Ala-[(S)-2-mercapto-pentanoyl]-Trp-NH2 is a research-grade reference peptide and is not approved by the FDA or any national regulator for human or veterinary therapeutic use. It is offered exclusively for in-vitro laboratory research and does not ship to the United States for consumer use.
In a protease substrates workflow, Ac-Pro-Leu-Ala-[(S)-2-mercapto-pentanoyl]-Trp-NH2 is reconstituted in sterile buffer or DMSO (solubility-dependent) and applied in the researcher's chosen assay format. Read on kinetic plate readers at the reporter-appropriate wavelength.
Other reference standards in the same class — commonly cross-referenced during assay design and comparator selection.