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Enzyme Substrates · Enzyme substrate (research reagent) · CASP-095

Ac-IETD-AFC

Research use · ships internationally
$85.00 / 1mg vial
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Ac-IETD-AFC reference vial
Drug class
Enzyme substrate (research reagent)
Status
Research use only
Catalog code
CASP-095
Also known as
CASP-095
Reviewed
2026-06-01 · Kindr Health Clinical Team

What Ac-IETD-AFC is studied for

  • Kinetic (kcat / Km) characterization of proteases and hydrolases
  • High-throughput inhibitor screening
  • Selectivity profiling across related enzyme families
  • Diagnostic and academic activity assays

Mechanism of action

Ac-IETD-AFC sits within the Enzyme Substrates research class (CPC reference CASP-095). The peptide encodes a recognition sequence flanked by a reporter group (pNA, AMC, MCA, FAM/DABCYL). Enzyme cleavage releases the reporter, producing a quantifiable optical signal proportional to catalytic activity. Investigators typically incorporate Ac-IETD-AFC as a reference standard, tool ligand, or substrate in the assay contexts listed above. Read on kinetic plate readers with the reporter-appropriate filter set (e.g. 405 nm, 380/460 nm).

Frequently asked questions

What is Ac-IETD-AFC?

Ac-IETD-AFC (CPC catalog code CASP-095) is a synthetic enzyme substrate (research reagent) in the enzyme substrates category. It is provided as a lyophilized ~1 mg vial for in-vitro research use only.

What is Ac-IETD-AFC studied for?

Published and internal research programs use Ac-IETD-AFC as a reference standard in enzyme substrates workflows — for example, kinetic (kcat / km) characterization of proteases and hydrolases and high-throughput inhibitor screening. Human clinical evidence is not established.

Is Ac-IETD-AFC FDA-approved for human use?

No. Ac-IETD-AFC is a research-grade reference peptide and is not approved by the FDA or any national regulator for human or veterinary therapeutic use. It is offered exclusively for in-vitro laboratory research and does not ship to the United States for consumer use.

How is Ac-IETD-AFC typically used in the lab?

In a enzyme substrates workflow, Ac-IETD-AFC is reconstituted in sterile buffer or DMSO (solubility-dependent) and applied in the researcher's chosen assay format. Read on kinetic plate readers with the reporter-appropriate filter set (e.g. 405 nm, 380/460 nm).

How should Ac-IETD-AFC be stored?

Store lyophilized at −20 °C, protected from light. DMSO stocks stable at −80 °C for months. Follow your institution's standard operating procedures for handling synthetic peptides.

Where does the Ac-IETD-AFC product page point to?

This kindr reference page mirrors CPC Scientific's catalog metadata for CASP-095. The upstream reference sheet — including sequence, purity, MW, and application notes where published — is linked from the buy panel. All orders placed on kindr Peptides ship internationally under research-use terms only.

What quality specifications apply to Ac-IETD-AFC?

Research-grade peptides in the CPC catalog are synthesized by solid-phase peptide synthesis (SPPS), purified by preparative RP-HPLC, and characterized by analytical HPLC and mass spectrometry. Typical purity is ≥95%; certificate of analysis is available on request.

Internal reference sheetOpen the Ac-IETD-AFC physicochemical & handling reference →Upstream reference sheetView CPC Scientific catalog page for CASP-095 →Add to cart · International research catalogBuy Ac-IETD-AFC in the kindr Peptides Shop →

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Research & medical disclosure. This page is educational reference content reviewed by a licensed physician. It is not medical advice and does not establish a clinician–patient relationship. Peptides offered on the kindr Peptides Shop are sold for in vitro laboratory research purposes only and ship internationally — not to the United States. For U.S. clinical care, use the kindr Longevity intake.