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Protease Substrates · Protease substrate (research reagent) · MMPS-025

NFF-2/NFF-3 Standard

Research use · ships internationally
$85.00 / 1mg vial
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NFF-2/NFF-3 Standard reference vial
Drug class
Protease substrate (research reagent)
Status
Research use only
Catalog code
MMPS-025
Also known as
MMPS-025
Reviewed
2026-06-01 · Kindr Health Clinical Team

What NFF-2/NFF-3 Standard is studied for

  • Kinetic (kcat / Km) protease characterization
  • Inhibitor screening and IC₅₀ determination
  • Selectivity profiling across protease families
  • Cell-based and diagnostic activity assays

Mechanism of action

NFF-2/NFF-3 Standard sits within the Protease Substrates research class (CPC reference MMPS-025). The peptide encodes a specific protease-recognition motif flanked by a reporter (pNA, AMC, MCA / Dnp FRET pair). Enzymatic cleavage releases the reporter, producing signal directly proportional to catalytic activity. Investigators typically incorporate NFF-2/NFF-3 Standard as a reference standard, tool ligand, or substrate in the assay contexts listed above. Read on kinetic plate readers at the reporter-appropriate wavelength.

Frequently asked questions

What is NFF-2/NFF-3 Standard?

NFF-2/NFF-3 Standard (CPC catalog code MMPS-025) is a synthetic protease substrate (research reagent) in the protease substrates category. It is provided as a lyophilized ~1 mg vial for in-vitro research use only.

What is NFF-2/NFF-3 Standard studied for?

Published and internal research programs use NFF-2/NFF-3 Standard as a reference standard in protease substrates workflows — for example, kinetic (kcat / km) protease characterization and inhibitor screening and ic₅₀ determination. Human clinical evidence is not established.

Is NFF-2/NFF-3 Standard FDA-approved for human use?

No. NFF-2/NFF-3 Standard is a research-grade reference peptide and is not approved by the FDA or any national regulator for human or veterinary therapeutic use. It is offered exclusively for in-vitro laboratory research and does not ship to the United States for consumer use.

How is NFF-2/NFF-3 Standard typically used in the lab?

In a protease substrates workflow, NFF-2/NFF-3 Standard is reconstituted in sterile buffer or DMSO (solubility-dependent) and applied in the researcher's chosen assay format. Read on kinetic plate readers at the reporter-appropriate wavelength.

How should NFF-2/NFF-3 Standard be stored?

Store lyophilized at −20 °C, protected from light. DMSO stocks stable at −80 °C. Follow your institution's standard operating procedures for handling synthetic peptides.

Where does the NFF-2/NFF-3 Standard product page point to?

This kindr reference page mirrors CPC Scientific's catalog metadata for MMPS-025. The upstream reference sheet — including sequence, purity, MW, and application notes where published — is linked from the buy panel. All orders placed on kindr Peptides ship internationally under research-use terms only.

What quality specifications apply to NFF-2/NFF-3 Standard?

Research-grade peptides in the CPC catalog are synthesized by solid-phase peptide synthesis (SPPS), purified by preparative RP-HPLC, and characterized by analytical HPLC and mass spectrometry. Typical purity is ≥95%; certificate of analysis is available on request.

Internal reference sheetOpen the NFF-2/NFF-3 Standard physicochemical & handling reference →Upstream reference sheetView CPC Scientific catalog page for MMPS-025 →Add to cart · International research catalogBuy NFF-2/NFF-3 Standard in the kindr Peptides Shop →

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Research & medical disclosure. This page is educational reference content reviewed by a licensed physician. It is not medical advice and does not establish a clinician–patient relationship. Peptides offered on the kindr Peptides Shop are sold for in vitro laboratory research purposes only and ship internationally — not to the United States. For U.S. clinical care, use the kindr Longevity intake.